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spsA [2018-11-19 19:10:43]
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spsA [2018-11-19 19:10:43]

nucleotide-sugar-dependent glycosyltransferase, spore coat polysaccharide synthesis
locus
BSU37910
pI
5.36
mw
30.03 kDa
protein length
256 aa Sequence Blast
gene length
768 bp Sequence Blast
function
spore coat polysaccharide synthesis
product
nucleotide-sugar-dependent glycosyltransferase
essential
no
synonyms
ipa-63d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    3,892,351 → 3,893,121

    Phenotypes of a mutant

  • reduced [SW|germination] efficiency [Pubmed|25239894]

    • The protein

    Protein family

  • glycosyltransferase 2 family [Pubmed|10350455]

    • Paralogous protein(s)

  • [protein|9EDE093896E8535962903D7A202D69C1BC402C09|CgeD]: the N-terminal 220 aa of [protein|9EDE093896E8535962903D7A202D69C1BC402C09|CgeD] and [protein|219B0AAFB4ABB7E97A906553B73423F712505E27|SpsA]: 45.5% identity

    • Structure

  • [PDB|1QGS] (complex with UDP) [Pubmed|10350455]
  • [PDB|1H7Q] [Pubmed|11733986]

    • Expression and Regulation

    Operons

    genes
    [gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]-[gene|9E577B935FAB9605DAF83353E907A6561F11CAC1|spsB]-[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]-[gene|2648D9E356683F5E9C00C40F0A6BBD1933322B09|spsD]-[gene|F27921CD9B89EB16AA57ACECEDBF21ED383629F0|spsE]-[gene|222F7086A214A427174E188CB1FEE4A8B98D303C|spsF]-[gene|9B34C9DBEC5B84A631CDFBEE8DF44384FF5C03B0|spsG]-[gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]-[gene|90D505CAA4FAAEB6A6AF5C278E70DF714FACB4DA|spsJ]-[gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]-[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|26577401], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|25239894,15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

    • regulation

  • expressed during [SW|sporulation] in the mother cell ([SW|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|26577401,25239894,15383836]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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    Biological materials

    Mutant

  • BKE37910 (Δ[gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATCCTACACCTCCTTCT, downstream forward: _UP4_AAAAAGCTTGGAATGGGGTG
  • BKK37910 (Δ[gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATCCTACACCTCCTTCT, downstream forward: _UP4_AAAAAGCTTGGAATGGGGTG

    • References

  • 9353933,15383836,11733986,10350455,25239894,26577401